2024
Hirsch, M., Desai, R.R., Annaswamy, S., Keatinge-Clay, A.T. (2024). Mutagenesis supports AlphaFold prediction of how modular polyketide synthase acyl carrier proteins dock with downstream ketosynthases. Proteins Link
Ray, K.A., Saif, N., Keatinge-Clay, A.T. (2024). Modular polyketide synthase ketosynthases collaborate with upstream dehydratases to install double bonds. Chem. Comm. Link
2023
Moor, S.R., Howard, J.R., Herrera, B.T., McVeigh, M.S., Marini, F., Keatinge-Clay, A.T., Anslyn, E.V. (2023). Determination of enantiomeric excess and diastereomeric excess via optical methods. Application to alpha-methyl-beta-hydroxy-carboxylic acids. Org. Chem. Front. 10: 1386-1392. Link
Keatinge-Clay, A.T., Miyazawa, T., Zhang, J., Ray, K.A., Lutgens, J.D., Bista, R., Lin, S.N. (2023). Crystal structures reveal the framework of cis-acyltransferase modular polyketide synthases. bioRxiv. doi: 10.1101/2023.02.11.528132. Link
2022
Dickinson, M.S., Miyazawa, T., McCool, R.S., Keatinge-Clay, A.T. (2022). Priming enzymes from the pikromycin synthase reveal how assembly line ketosynthases catalyze carbon-carbon chemistry. Structure 30:1331-1339. Link
2021
Hirsch, M., Fitzgerald, B.J., Keatinge-Clay, A.T. (2021). How cis-acyltransferase assembly-line ketosynthases gatekeep for processed polyketide intermediates. ACS Chem. Biol. 16:2515-2526. Link
Miyazawa, T., Fitzgerald, B.J., Keatinge-Clay, A.T. (2021). Preparative production of an enantiomeric pair by engineered polyketide synthases. Chem. Commun. 57:8762-8765. Link
Hirsch, M., Kumru,K., Desai, R.R., Fitzgerald, B.J., Miyazawa, T. Ray, K.A., Saif, N., Spears, S., Keatinge-Clay, A.T. (2021). Insights into modular polyketide synthase loops aided by repetitive sequences. Proteins 89:1099-1110. Link
2020
2019
Meinke, J.L., Simon, A.J., Wagner, D.T., Morrow, B.R., You, S., Ellington, A.D., Keatinge-Clay, A.T. (2019). Employing 25-residue docking motifs from modular polyketide synthases as orthogonal protein connectors. ACS Synthetic Biology 8:2017-2024. Link
Valencia, L.E., Zhang, Z., Cepeda, A.J., Keatinge-Clay, A.T. (2019). Seven-enzyme in vitro cascade to (3R)-3-hydroxybutyryl-CoA. Org. Biomol. Chem. 17:1375-1378. Link
2018
Liu, C., Yuan, M., Xu, X., Wang, L., Keatinge-Clay, A.T., Deng, Z., Lin, S., Zheng, J. (2018). Stereochemical insights from substrate-bound structures of ketoreductases from modular polyketide synthases. J. Struct. Biol. 203:135-141. Link
Vander Wood, D.A., Keatinge-Clay, A.T. (2018). The modules of trans-acyltransferase assembly lines redefined with a central acyl carrier protein. Proteins 86:664-675. Link
Wagner, D.T., Zhang, Z., Meoded, R.A., Cepeda, A.J., Piel, J., Keatinge-Clay, A.T. (2018). Structural and functional studies of a pyran synthase domain from a trans-acyltransferase assembly line. ACS Chem. Biol. 13:975-983. Link
2017
Keatinge-Clay, A.T. (2017). Polyketide synthase modules redefined. Angew. Chem. Int. Ed. Engl. 56:4658-4660. Link
2016
Wagner, D.T., Stevens, D.C., Mehaffey, R.M., Manion, H.R., Taylor, R.E., Brodbelt, J.S., Keatinge-Clay, A.T. (2016). alpha-Methylation follows condensation in the gephyronic acid modular polyketide synthase. Chem. Commun. 52:8822-8825. Link
Keatinge-Clay, A.T. (2016). The structural relationship between iterative and modular polyketide synthases. Cell Chem. Biol. 23:540-542. Link
Xie, X., Garg, A., Keatinge-Clay, A.T., Khosla, C., Cane, D.E. (2016). Epimerase and reductase activities of polyketide synthase ketoreductase domains utilize the same conserved tyrosine and serine residues. Biochemistry 55:1179-1186. Link
Gay, D.C., Wagner, D.T., Meinke, J.L., Zogzas, C.E., Gay, G.R., Keatinge-Clay, A.T. (2016). The LINKS motif zippers trans- acyltransferase polyketide synthase assembly lines into a biosynthetic megacomplex. J. Struct. Biol. 193:196-205. Link
Keatinge-Clay, A.T. (2016). Stereocontrol within polyketide assembly lines. Nat. Prod. Rep. 33:141-149. Link
2015
Fage, C.D., Meinke, J.L., Keatinge-Clay, A.T. (2015). Coenzyme A-free activity, crystal structure, and rational engineering of a promiscuous beta-ketoacyl thiolase from Ralstonia eutropha. J. Mol. Catal. B: Enzym. 121:113-121. Link
Mugnai, M.L., Shi, Y., Keatinge-Clay, A.T., Elber, R. (2015). Molecular dynamics studies of modular polyketide synthase ketoreductase stereospecificity. Biochemistry 54:2346-2359. Link
Fage, C.D., Isiorho, E.A., Liu, Y., Wagner, D.T., Liu, H.W., Keatinge-Clay, A.T. (2015). The structure of SpnF, a standalone enzyme that catalyzes [4 + 2] cycloaddition. Link Nat. Chem. Biol. 11:256-258.
Lee, J., Monzingo, A.F., Keatinge-Clay, A.T., Harshey, R.M. (2015). Structure of Salmonella FlhE, conserved member of a flagellar type III secretion operon. J. Mol. Biol. 427:1254-1262. Link
2014
Garg, A., Xingiang, X., Keatinge-Clay, A.T., Khosla, C., Cane, D.E. (2014). Elucidation of the cryptic epimerase activity of redox-inactive ketoreductase domains from modular polyketide synthases by tandem equilibrium isotope exchange. J. Am. Chem. Soc. 136:10190-10193. Link
Gay, G., Wagner, D.T., Keatinge-Clay, A.T., Gay, D.C. (2014). Rapid modification of the pET-28 expression vector for ligation independent cloning using homologous recombination in Saccharomyces cerevisiae. Plasmid pii: S0147-619X(14)00075-4. Link
Fage, C.D., Henderson, J.C., Cannon, J.R., Brodbelt, J.S., Keatinge-Clay, A.T., Trent, M.S. (2014). Antimicrobial peptide resistance of Vibrio cholerae results from LPS modification pathway related to non-ribosomal peptide synthetases. ACS Chem. Biol. 9:2382-2392. Link
Isiorho, E.A., Jeon, B.-S., Liu, H.-w., Keatinge-Clay, A.T. (2014) Structural studies of the spinosyn forosaminyltransferase, SpnP. Biochemistry 53:4292-4301. Link
Fage, C.D., Brown, D.B., Boll, J.M., Keatinge-Clay, A.T., Trent, M.S. (2014). Crystallographic study of the EptC phosphoethanolamine transferase required for polymyxin resistance and motility in Campylobacter jejuni. Acta Cryst. D 70:2730-2739. Link
Brantley, J.N., Bailey, C.B., Cannon, J.R., Clark, K.A., Vanden Bout, D.A., Brodbelt, J.S., Keatinge-Clay, A.T., Bielawski, C.W. (2014). Mechanically modulating the photophysical properties of fluorescent protein biocomposites: new classes of ratio- and intensiometric sensors. Angew. Chem. 53:5088-5098. Link
Piasecki, S.K., Zheng, J., Axelrod, A.J., Detelich, M., Keatinge-Clay, A.T. (2014). Structural and functional studies of a trans-acyltransferase polyketide synthase ketoreductase the performs both alpha- and beta-ketoreduction. Proteins 82:2067-2077. Link
Gay, D.C., Gay, G., Axelrod, A.J., Jenner, M., Kohlhaas, C., Kampa, A., Oldham, N.J., Piel, J., Keatinge-Clay, A.T. (2014). A close look at a ketosynthase from a trans-acyltransferase modular polyketide synthase. Structure 22:444-451. Link
Hughes, A.J., Tibby, M.R., Wagner, D.T., Brantley, J.N., Keatinge-Clay, A.T. (2014). Investigating the reactivities of a polyketide synthase module through fluorescent click chemistry. Chem. Commun. 50: 5276-5278. Link
2013
Enyeart, P.J., Chirieleison, S.M., Dao, M.N., Perutka, J., Quandt, E.M., Yao, J., Whitt, J.T., Keatinge-Clay, A.T., Lambowitz, A.M., Georgiou, G., Ellington, A.D. (2013). Generalized bacterial genome editing using mobile group II introns and Cre-lox. Mol. Syst. Biol. 9: 685-700. Link
Zheng, J., Piasecki, S.K., Keatinge-Clay, A.T. (2013). Structural studies of an A2-type modular polyketide synthase ketoreductase reveal features controlling alpha-substituent stereochemistry. ACS Chem. Biol. 8:1964-1971. Link
Zheng, J., Fage, C.D., Demeler, B., Hoffman, D.W., Keatinge-Clay, A.T. (2013). The missing linker: a dimerization motif located within polyketide synthase modules. ACS Chem. Biol. 8:1263-1270. Link
Brantley, J.N., Bailey, C.B., Wiggins, K.M., Keatinge-Clay, A.T., Bielawski, C.W. (2013). Mechanobiochemistry: harnessing biomacromolecules for force-responsive materials. Polym. Chem. 4:3916-3928. Link
2012
Keatinge-Clay, A.T. (2012). The structures of type I polyketide synthases. Nat. Prod. Rep. 29:1050-1073. Link
Piasecki, S.K. & Keatinge-Clay, A.T. (2012). Monitoring biocatalytic transformations mediated by polyketide synthase enzymes via fluorine NMR. Synlett 12:1840-1842. Link
Zheng, J., Gay, D.C., Demeler, B., White, M.A., Keatinge-Clay, A.T. (2012). Divergence of multimodular polyketide synthases revealed by a didomain structure. Nat. Chem. Biol. 8:615-621. Link
Hughes, A.J. & Keatinge-Clay, A.T. (2012). Employing a polyketide synthase module and thioesterase in the semipreparative biocatalysis of diverse triketide pyrones. Med. Chem. Commun. 3:956-959. Link
Isiorho, E., Liu, H.-w., Keatinge-Clay, A.T. (2012). Structural studies of the spinosyn rhamnosyltransferase, SpnG. Biochemistry 51:1213-1222. Link
2011
Zheng, J. & Keatinge-Clay, A.T. (2011). Structural and functional analysis of C2-type ketoreductases from modular polyketide synthases. J. Mol. Biol. 410:105-117. Link
Hughes, A.J. & Keatinge-Clay, A.T. (2011). Enzymatic extender unit generation for in vitro polyketide synthase reactions: structural and functional showcasing of Streptomyces coelicolor MatB. Chem. Biol. 18:165-176. Link
2010
Zheng, J., Taylor, C.A., Piasecki, S.K., Keatinge-Clay, A.T. (2010). Structural and functional analysis of A-type ketoreductases from the amphotericin modular polyketide synthase. Structure 18:913-922. Link
2008
Castonguay, R., Valenzano, C.R., Chen, A.Y., Keatinge-Clay, A.T., Khosla, C., Cane, D.E. (2008). Stereospecificity of ketoreductase domains 1 and 2 of the tylactone modular polyketide synthase" J. Am. Chem. Soc. 130:11598-11599. Link