Our lab helped redefine the PKS module and is now using this updated definition to engineer modular PKSs that efficiently produce novel polyketides! Check out our science through YouTube or our publications:

Keatinge-Clay, A.T. (2017). Polyketide synthase modules redefined. Angew. Chem. Int. Ed. Engl. 56:4658-4660. Link

Vander Wood, D.A., Keatinge-Clay, A.T. (2018). The modules of trans-acyltransferase assembly lines redefined with a central acyl carrier protein. Proteins 86:664-675. Link

Miyazawa, T., Hirsch, M., Zhang, Z., Keatinge-Clay, A.T. (2020). An in vitro platform for engineering and harnessing modular polyketide synthases. Nat. Commun. 1:80. Link

Miyazawa, T., Fitzgerald, B.J., Keatinge-Clay, A.T. (2021). Preparative production of an enantiomeric pair by engineered polyketide synthases. Chem. Commun. 57:8762-8765. Link

Hirsch, M., Fitzgerald, B.J., Keatinge-Clay, A.T. (2021). How cis-acyltransferase assembly-line ketosynthases gatekeep for processed polyketide intermediates. ACS Chem. Biol. 16:2515-2526. Link

Zhang, J., Bista, R., Miyazawa, T., Keatinge-Clay, A.T. (2023) Boosting titers of engineered triketide and tetraketide synthases to record levels through T7 promoter tuning. Metab. Eng. 78:93-98. Link

Ray, K.A., Saif, N., Keatinge-Clay, A.T. (2024). Modular polyketide synthase ketosynthases collaborate with upstream dehydratases to install double bonds. Chem. Comm. Link

Hirsch, M., Desai, R.R., Annaswamy, S., Keatinge-Clay, A.T. (2024). Mutagenesis supports AlphaFold prediction of how modular polyketide synthase acyl carrier proteins dock with downstream ketosynthases. Proteins Link

Ray, K.A., Lutgens, J.D., Bista, R., Zhang, J., Desai, R.R., Hirsch, M., Miyazawa, T., Cordova, A., Keatinge-Clay, A.T. (2024). Assessing and harnessing updated polyketide synthase modules through combinatorial engineering. Nat. Commun. Link